Peptide Separation by Two-dimensional Chromatography and Electrophoresis

High-resolution separation of cardiovascular proteomes: two-dimensional electrophoresis and liquid chromatography.

Rapid peptide separation by supercritical fluid chromatography.

BACKGROUND Supercritical fluid chromatography (SFC) is continually gaining attention in the separation sciences as demand increases for higher throughput isolations and purifications. The higher flow rates associated with SFC provide a significant decrease in analysis time and increase in sample-throughput efficiency. Peptides are of particular interest for SFC due, in part, to the rather exten...

Immunogens of Brucella Abortus S19 Identified By Two-Dimensional Gel Electrophoresis and Immunoblotting

Background: Lipopolysaccharides (LPSs) and several antigenic proteins of Brucella have been considered for preparation of diagnostic reagents and subunit vaccines. The objective of this study was to identify and compare immunogens of B. abortus S19 which induce humoral immune responses in human, goat and rabbit. Material and Methods: The bacterial whole cell extract was prepared in extraction b...

Simulation and optimization of peptide separation by capillary electrophoresis.

The different mobility equations that have appeared in the literature for predicting peptide mobility were compared. A modified equation that relates the mobility of individual proteolytic species of a peptide to its composition has been obtained: mu = 1758 log(1 + 0.297q)/M0.411, where mu is the electrophoretic mobility in 10(-9) m2/s.V, q is the integral value of the charge of the species and...

Separation and identification of soybean leaf proteins by two-dimensional gel electrophoresis and mass spectrometry.

To establish a proteomic reference map for soybean leaves, we separated and identified leaf proteins using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry (MS). Tryptic digests of 260 spots were subjected to peptide mass fingerprinting (PMF) by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) MS. Fifty-three of these protein spots were id...